Arch Iran Med. 2024;27(6): 305-312.
doi: 10.34172/aim.28579
  Abstract View: 149
  PDF Download: 88

Original Article

Low Prevalence of Anti-HBc Antibody and Lack of HBV DNA Among HBsAg-Negative Blood Donors in Iran: A Cross-sectional Study and Review of Literature

Mohammad Reza Hedayati-Moghaddam 1 ORCID logo, Farahnaz Tehranian 2,3, Arman Mosavat 1 ORCID logo, Rahele Miri 1, Sanaz Ahmadi Ghezeldasht 1* ORCID logo

1 Blood Borne Infections Research Center, Academic Center for Education, Culture and Research (ACECR), Razavi Khorasan, Mashhad, Iran
2 Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
3 Razavi Khorasan Blood Transfusion Center, Mashhad, Iran
*Corresponding Author: Sanaz Ahmadi Ghezeldasht, Email: snz855951@gmail.com


Background: Occult hepatitis B infection (OBI) refers to the presence of hepatitis B virus (HBV) DNA in the serum or liver of individuals who tested negative for HBV surface antigen (HBsAg). This study aimed to determine seropositivity for antibodies against HBV core antigen (anti-HBc) and the frequency of OBI among the HBsAg non-reactive blood donors in Mashhad, northeastern Iran.

Methods: In this cross-sectional study, serum samples of HBsAg-negative blood donors were examined for anti-HBc during June and August 2018. Anti-HBc-positive samples were tested for antibodies against HBsAg (anti-HBs), and those with negative results were classified as isolated anti-HBc cases. The presence of HBV DNA in the C, S, and X gene regions was assessed by a qualitative real-time polymerase chain reaction method in all HBsAg-negative samples. OBI subjects were detected by the presence of at least one HBV genomic region.

Results: Of 540 HBsAg-negative donors, 29 (5.4%; 95% confidence interval: 3.6–7.6%) showed seroreactivity for anti-HBc, of whom 18 individuals were also seropositive for anti-HBs. All donors showed negative results for all three HBV genes regardless of their serum anti-HBc status.

Conclusion: Based on our findings, we suggest routine screening of Iranian blood donation volunteers for serum anti-HBc and anti-HBs but not HBV DNA.

Cite this article as: Hedayati-Moghaddam MR, Tehranian F, Mosavat A, Miri R, Ahmadi Ghezeldasht S. Low prevalence of anti-HBc antibody and lack of HBV DNA among HBsAg-negative blood donors in Iran: a cross-sectional study and review of literature. Arch Iran Med. 2024;27(6):305-312. doi: 10.34172/aim.28579
First Name
Last Name
Email Address
Security code

Abstract View: 150

Your browser does not support the canvas element.

PDF Download: 88

Your browser does not support the canvas element.

Submitted: 29 Nov 2023
Accepted: 11 Mar 2024
ePublished: 27 Apr 2024
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)