Logo-aim
Arch Iran Med. 2022;25(12): 807-816.
doi: 10.34172/aim.2022.126
PMID: 37543908
PMCID: PMC10685841
Scopus ID: 85158081228
  Abstract View: 795
  PDF Download: 538

Original Article

Iron Oxide Nanoparticles Conjugated to Thiosemicarbazone Reduce the Survival of Cancer Cells by Increasing the Gene Expression of MicroRNA let-7c in Lung Cancer A549 Cells

Alireza Habibi 1* ORCID logo, Nesa Bakhshi 2, Zeinab Moradi shoili 3, Nour Amirmozafari 4 ORCID logo

1 Departman of Basic Sciences, Faculty of Science, Imam Hossein University, Tehran, Iran
2 Departman of Molecular Cell Biology, Faculty of Science, Islamic Azad University of Lahijan, Lahijan, Iran
3 Departman of Chemistry, Faculty of Science, University of Guilan, Rasht, Iran
4 Departman of Microbiology, School of Medicine, University of Medical Sciences, Tehran, Iran
*Corresponding Author: Alireza Habibi,, Email: Alirh110@gmail.com

Abstract

Background: Cancer cells have a higher demand for iron to grow and proliferate. A new complex of iron nanoparticles and thiosemicarbazones was synthesized. Confirmation tests included UV-visible, scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), Fourier transform infrared (FTIR), X-ray diffraction (XRD) and zeta potential.

Methods: MTT assay, flow cytometry and qRT-PCR were used to investigate anti-proliferative effect, amount of apoptosis and the effect of Fe3 O4 @Glu/BTSC on changes in gene expression of microRNA let-7c (let-7c), respectively. The specifications of Fe3 O4 @ Glu/BTSC were confirmed at 5 nm.

Results: Fe3O4@Glu/BTSC was more effective than BTSC and Fe3 O4 on A549 cells (IC50=166.77 µg/mL) but its effect on healthy cells was smaller (CC50=189.15 µg/mL). The drug selectivity index (SI) was calculated to be 1.13. The initial apoptosis rate was 46.33% for Fe3 O4 @Glu/BTSC, 28.27% for BTSC and 26.02% for Fe3 O4 . BTSC and BTSC@Fe3 O4 inhibited the cell cycle progression in the Sub-G1 and S phases. let-7c expression was 6.9 times higher in treated cells compared to the control group. The expression rate was 2.2 with BTSC compared to the control group and 1.6 times for Fe3 O4.

Conclusion: Fe3 O4 @Glu/BTSC has proper anti-proliferative effects against lung cancer cells by increasing the expression of let-7c and inhibiting the cell cycle with the apoptosis activation pathway.


Cite this article as: Habibi A, Bakhshi N, Moradi Shoili Z, Amirmozafari N. Iron oxide nanoparticles conjugated to thiosemicarbazone reduce the survival of cancer cells by increasing the gene expression of MicroRNA let-7c in lung cancer a549 cells. Arch Iran Med. 2022;25(12):807-816. doi: 10.34172/aim.2022.126
First Name
Last Name
Email Address
Comments
Security code


Abstract View: 796

Your browser does not support the canvas element.


PDF Download: 538

Your browser does not support the canvas element.

Submitted: 24 Aug 2021
Revision: 26 Oct 2021
Accepted: 07 Nov 2021
ePublished: 01 Dec 2022
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)