Academy of Medical Sciences of I.R. Iran Archives of Iranian Medicine 1029-2977 26 2 2023 02 01 Prevalence of Chromosomal Abnormalities in Iranian Patients with Infertility 110 116 10.34172/aim.2023.17 EN Saima Abbaspour https://orcid.org/0009-0002-1867-4364 Alireza Isazadeh https://orcid.org/0000-0002-8781-1177 Matin Heidari Masoud Heidari Saba Hajazimian Morteza Soleyman-Nejad Mohammad Hossein Taskhiri Manzar Bolhassani Amir Hossein Ebrahimi Parvaneh Keshavarz Zahra Shiri Mansour Heidari https://orcid.org/0000-0001-6394-0970 Journal Article 10.34172/aim.2023.17 2022 06 13 2022 09 13 Background: The numerical and structural abnormalities of chromosomes are the most common cause of infertility. Here, we evaluated the prevalence and types of chromosomal abnormalities in Iranian infertile patients. Methods: We enrolled 1750 couples of reproductive age with infertility, who referred to infertility clinics in Tehran during 2014- 2019, in order to perform chromosomal analysis. Peripheral blood samples were obtained from all couples and chromosomal abnormalities were evaluated by G-banded metaphase karyotyping. In some cases, the detected abnormalities were confirmed using fluorescence in-situ hybridization (FISH). Results: We detected various chromosomal abnormalities in 114/3500 (3.257%) patients with infertility. The prevalence of chromosomal abnormalities was 44/114 (38.596%) among infertile females and 70/114 (61.403%) among infertile males. Structural chromosomal abnormalities were found in 27/1750 infertile females and 35/1750 infertile males. Numerical chromosomal abnormalities were found in 17/1750 of females and 35/1750 of males. The 45, XY, rob (13;14) (p10q10) translocation and Klinefelter syndrome (47, XXY) were the most common structural and numerical chromosomal abnormalities in the Iranian infertile patients, respectively. Conclusion: In general, we found a high prevalence of chromosomal abnormalities in Iranian patients with reproductive problems. Our study highlights the importance of cytogenetic studies in infertile patients before starting infertility treatments approaches.