Logo-aim
Arch Iran Med. 2020;23(9): 593-599.
doi: 10.34172/aim.2020.69

Scopus ID: 85091809523
  Abstract View: 2190
  PDF Download: 1205

Original Article

Antiproliferative Effects of Recombinant Apoptin on Lung and Breast Cancer Cell Lines

Masoud Ezami Razliqi 1 ORCID logo, Gholamreaza Olad 2*, Rouhollah Dorostkar 1, Sahar Heydari 2, Hadi Esmaeili Gouvarchin Ghaleh 1

1 Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
2 Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
*Corresponding Author: *Corresponding Author: Gholamreaza Olad, PhD; Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Tel: +98- 9128043492. Email: , Email: grolad@gmail.com

Abstract

Background: Selective therapy has always been the main challenge in cancer treatments. Various non-replicative oncolytic viral systems have revealed the safety and efficacy of using viruses and these products. The aim of this paper is to examine the impact of recombinant apoptin on the proliferation of lung cancer and breast cancer cell lines.

Methods: The present study consisted of two steps of expression of recombinant apoptin and its anti-proliferative effects on normal and cancer cells. In the first step, following bioinformatics and optimizing apoptin gene sequencing and synthesis, it was expressed using vector PET28a and E. coli BL21 (DE3). The expressed recombinant apoptin was confirmed by analytical SDSPAGE and then purified using Ni affinity chromatography. In the second step, the antiproliferative effects of recombinant apoptin on lung cancer, breast cancer and primary cell lines were determined using MTT assay.

Results: According to the results of SDS-PAGE gel assay, recombinant apoptin was visible in the 14 kDa band. Also, the MTT assay results indicated that the antiproliferative effects of recombinant apoptin in cancer cell lines was different compared with the primary cell line, and followed a dose-dependent manner in both cell lines. The highest cytotoxicity (lowest cell viability) groups were 0.2 mg/mL in lung cancer (0.32 ± 0.015) (P<0.001), and in breast cancer (0.33 ± 0.031) (P<0.001) and 0.032 mg/mL in primary cells (0.17 ± 0.004) (P<0.01), as compared to the control groups.

Conclusion: Our results confirmed that recombinant apoptin can induce antiproliferative effects in lung cancer and breast cancer cell lines, but not in normal monkey kidney cell line Vero; thus, it can be introduced as a promising novel specific antitumor agent after further evaluation in clinical trials.


Cite this article as: Ezami Razliqi M, Olad G, Dorostkar R, Heydari S, Esmaeili Gouvarchin Ghaleh H. Antiproliferative effects of recombinant apoptin on lung and breast cancer cell lines. Arch Iran Med. 2020;23(9):593–599. doi: 10.34172/aim.2020.69.
First Name
Last Name
Email Address
Comments
Security code


Abstract View: 2191

Your browser does not support the canvas element.


PDF Download: 1205

Your browser does not support the canvas element.

Submitted: 11 Dec 2019
Accepted: 18 Feb 2020
ePublished: 01 Sep 2020
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)